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	Provedor de dados Genet. Mol. Biol. (3) Autor Morgante,João Stenghel (3) Dantas,Gisele Pires de Mendonça (1) Ditchfield,Albert (1) Ferrand,Nuno (1) Ferreira,Juliana Machado (1) Godinho,Raquel (1) Martins,Felipe de Melo (1) Meyer,Diogo (1) Moraes-Barros,Nadia de (1) Mais... Palavra-chave Bradypus (1) Cytb (1) DNA (1) Dried skins (1) FIB7 intron (1) Identification (1) Museum specimens (1) PCR-RFLP (1) Phyllostomidae (1) Phylogeography (1) Platyrrhinus (1) Recent origin (1) Selective sweep (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2012 (1) 2007 (1) 2005 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última A simple protocol for the extraction and sequence analysis of DNA from study skin of museum collections Genet. Mol. Biol. Moraes-Barros,Nadia de; Morgante,João Stenghel. Museum collections have been widely used as sources of biological samples for molecular biology studies and there are several methodologies and techniques to obtain and analyze DNA from tissues archived in museums, but most of these protocols have been developed for a specific tissue or are commercial kits. We present a simple protocol for extracting and amplifying DNA segments from sloth museum specimens. With this simple protocol we analyzed DNA fragments from 64% of 64 skin samples from three-toed sloths (Bradypus variegatus and Bradypus tridactylus) archived in three different museums: 43 samples from the University of São Paulo Museum of Zoology (Museu de Zoologia da Universidade de São Paulo, MUZUSP) São Paulo, São Paulo, Brazil; 18 samples from the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradypus; DNA; Dried skins; Museum specimens. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600024 Genetic variability in mitochondrial and nuclear genes of Larus dominicanus (Charadriiformes, Laridae) from the Brazilian coast Genet. Mol. Biol. Dantas,Gisele Pires de Mendonça; Meyer,Diogo; Godinho,Raquel; Ferrand,Nuno; Morgante,João Stenghel. Several phylogeographic studies of seabirds have documented low genetic diversity that has been attributed to bottleneck events or individual capacity for dispersal. Few studies have been done in seabirds on the Brazilian coast and all have shown low genetic differentiation on a wide geographic scale. The Kelp Gull is a common species with a wide distribution in the Southern Hemisphere. In this study, we used mitochondrial and nuclear markers to examine the genetic variability of Kelp Gull populations on the Brazilian coast and compared this variability with that of sub-Antarctic island populations of this species. Kelp Gulls showed extremely low genetic variability for mitochondrial markers (cytb and ATPase) and high diversity for a nuclear locus (intron... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytb; FIB7 intron; Phylogeography; Recent origin; Selective sweep. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500023 The use of PCR-RFLP as an identification tool for two closely related species of bats of genus Platyrrhinus Genet. Mol. Biol. Ferreira,Juliana Machado; Martins,Felipe de Melo; Ditchfield,Albert; Morgante,João Stenghel. The bat species Platyrrhinus lineatus and P. recifinus (Phyllostomidae: Stenodermatinae) are ecologically important because of their capacity for seed dispersal. P. recifinus is endemic to the Atlantic rain forest and is considered vulnerable by the IUCN. The lack of distinct morphological features makes identification of the two species a difficult task. This study was aimed at testing the hypothesis that these are actually two distinct species by using PCR-RFLP of the mitochondrial cytocrome b gene. The results showed no shared haplotypes, demonstrating that these are, in fact, two distinct species. No polymorphism was obtained for P. recifinus, which could be a sign of low genetic diversity in this threatened species. Tipo: Info:eu-repo/semantics/article Palavras-chave: Platyrrhinus; PCR-RFLP; Identification; Phyllostomidae. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100021 Registros recuperados: 3 Primeira ... 1 ... Última

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